摘要
Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants.
Under stress conditions such as drought, high-salinity and low-temperature, the transcription factor of DREB （dehydration responsive element binding proteins） improved efficiently stress resistance by regulating the expression of its downstream genes with various environmental stress resistance in plants. GmDREB gene （GenBank Accession No. AF514908） encoding a stress-inducible transcription factor was cloned by screening a cDNA library of Glycine max cv. Jinong 27 with yeast one-hybrid method. GmDREB gene was 910 bp in length and encoded 174 amino acids containing a conserved AP2/EREBP DNA-binding domain of 58 amino acids. Two conserved functional amino acids, valine and glutamic acid, were located on the 14th and the 19th amino acid residues in the conserved structural domain. An alkaline amino acid region （KKR） related to a nuclear localization signal was at the N-terminal, while an acidic amino acid region （DDD） related to trans-activation was at the C-terminal. Plant expression vectors were constructed and transformed into wheat by bombardment. In total, 13 trans- genic plants with Ubi∷GmDREB and 11 transgenic plants with rd29A∷GmDREB were identified from 103 regeneration plants by molecular analysis. The drought and salt tolerances of T1 transgenic lines with Ubi∷GmDREB or rd29A∷GmDREB were demonstrated to be improved as compared to wild type. The result also suggested that both Ubiquitin and rd29A promoters could effectively drive the expression of the GrnDREB gene and enhance drought and salt tolerance of T1 plants.
基金
This work was supported by the National 863 Project(Grant No.2002AA224081)
National Special Project for Plant Transgenic and Industry(Grant No.JY03-A-18).
