咪唑安定对大鼠局灶性脑梗死的保护作用

Midazolam Has the Protective Effect on Local Cerebral Infarction in Rats

目的探讨咪唑安定是否具有脑保护作用。方法 SD雄性大鼠24只,用光化学法制作脑梗死模型。根据给药剂量、方法的不同将动物随机分为4组。咪唑安定1、2、3组分别腹腔注射咪唑安定0．5 mg/kg、1 mg/kg、2 mg/kg;对照组,腹腔注射生理盐水。从术前24 h开始注射,每8 h 1次,直至处死。术后动物存活24 h,然后在深麻下灌流固定、取脑、切片。在尼氏染色的切片上计算最大脑梗死面积,用TUNEL染色计数梗死区凋亡细胞数。结果咪唑安定3组最大脑梗死面积和TUNEL阳性细胞数明显少于其他3组(P<0．01);咪唑安定2组最大脑梗死面积和TUNEL阳性细胞数明显少于对照组( P<0．05);咪唑安定1组最大脑梗死面积和TUNEL阳性细胞数和对照组相比无明显差别。结论咪唑安定可以明显减少大鼠光化学脑梗死面积,咪唑安定在较大剂量时具有明显的脑保护作用。

Objective To investigate whether midazolam has the cerebral protective effect.Methods Twenty-four SD male rats were divided into 4 groups randomly, each 6. The rats in Midazolam group 1,2,3 were respectively received midazolam 0.5 mg/kg, 1 mg/kg,2 mg/kg intra-peritoneally. Control group was replaced by saline. From 24 h before surgery,all animals were received injection every 8 h until 24 h post-operation sacrificed Photothrombosis was used to produce a local cerebral ischemia in the parietal cortex in the all rats. The rats in all groups were sacrificed by perfusion 24 h after surgery. The maximum infarcted areas were calculated in Nissl-stained cortical sections. The numbers of TUNEL positive cells in the infracted areas were counted in TUNEL stained sections. Results The maximum infarction areas in Midazolam group 3 were smaller than that in other groups. The numbers of TUNEL positive cells in Midazolam groups 3 were also less than other groups＇ （ P 〈 0.01） .The maximum infarction areas and the numbers of TUNEL positive cells in Midazolam group 2 were smaller than that in control group （ P 〈 0.01） ;Midazolam group 1 compared with control group had no significant difference. Conclusion Midazolam can significantly decrease the cerebral infarction areas caused by photothrombosis in rats. Midazolam has cerebral protective effect and much dose of midazolam （2 mg/kg） can obviously produce cerebral protective action.