内蒙古糖甜菜叶斑病菌的多样性分析

Genetic diversity of Curtobacterium flaccumfaciens pv. beticola from Inner Mongolia in China

采用ERIC-PCR,BOX-PCR和ITS的分析方法,对分离自我国内蒙古自治区5个市的21个糖甜菜叶斑病菌菌株进行多样性分析,并与其他12种病原细菌进行比较。ERIC-PCR揭示,在相似性80%上所有参试菌株分为26簇,而BOX-PCR只得到20个簇,暗示这两种短重复序列在基因组中的分布不同;将两者电泳图谱结合,得到介于上述两者间的结果,分为23个簇;在相似率达87%时,ITS分析将21个糖甜菜叶斑病菌菌株分成7簇。3种分析方法相互验证,均说明内蒙古糖甜菜叶斑病菌基因组存在显著多样性。ERIC和BOX聚类证明了糖甜菜叶斑病菌与短小杆菌属(Curtobacterium)亲缘关系较近,与其他属细菌亲缘关系较远。研究证明,ERIC和BOX扩增基因组DNA指纹比ITS图谱具有更强的多样性。

Twenty-one strains of Curtobacterium flaccumfaciens pv. beticola collected from five cities in Inner Mongolia of China were analyzed by genotypic typing methods, including ERIC-PCR, BOX-PCR and ITS profiling （PCR ribotyping）. The results from rep -PCR with the two primers demonstrated that these strains of Cfb could be divided into 23 groups with 80% similarity to each other. ITS profiling of the 16S-23S rDNA spacer region gave 7 clusters at a level of 87% similarity based on dendrogram analysis. In summary, the results revealed conspicuous heterogeneity among the strains of C. flaccumfaciens pv. beticola that have been analyzed. It was suggested that ERIC-PCR and BOX-PCR were highly discriminative for detecting genetic variation among the strains, identifying the strains as well as classifying them.