水稻、小麦和油菜种子萌发POD与CAT对酸雨胁迫的响应

Response of POD and CAT During Seeds of Rice,Wheat and Rape Germination on Acid Rain Stress

研究了pH 2.5～5.0模拟酸雨对水稻(O.sativa)、小麦(T.aestivum)和油菜(B.chinensis var.oleifera)种子萌发过程中POD、CAT活性影响.静态与动态实验结果表明,酸雨胁迫下,3类种子萌发时CAT与POD活性的变化幅度是水稻(28.8%,31.7%)＜小麦(34.7%,48.3%)＜油菜(79.3%,50.0%);CAT与POD与CK达到差异显著水平(p＜0.05)的胁迫强度是水稻(pH3.5)＞小麦(pH4.0)＞油菜(pH5.0),说明保护酶抗酸雨能力是水稻＞小麦＞油菜.相同胁迫强度下,水稻、小麦和油菜种子CAT变幅＜POD,说明前者对酸雨胁迫敏感性大于后者.保护酶应激清除自由基反应能力的差异是决定3类种子萌发指标趋异的又一深层原因.

The effects of simulated acid rain （pH2.5- 5.0） on the activities of POD and CAT were investigated during germination of rice （O. sativa ）, wheat （ T. aestivurn ） and rape （ B. chinensis var. oleifera ） seeds. Compared with the control （CK）, the amplitude of the change in the activity of CAT and POD is that rice （28.8 %, 31.7 % ） 〈 wheat （34.7 %, 48.3 %, ） 〈 rape （79.3 %, 50.0 % ）, respectively. The pH significantly different （ p 〈 0.05 ） from CK follows the order： rice （3.5） 〉 wheat （ 4.0 ） 〉 rape （5.0）. All of these revealed that the ability of resisting acid rain stress is that rice is stronger than wheat and rape is the worst. Under the same condition, the amplitude of the change in the activity of CAT is more sensitivity toward acid rain stress than that of POD. The difference in free radicals removed by these 3 species is one of the reasons why the germinating indexes behaved differently.