摘要
应用反向高效液相色谱,以羟丙基-β-环糊精(HP--βCD)作为手性流动相添加剂,拆分了氯噻酮(Chlorthalidone)对映体。对主要的影响因素如羟丙基-β-环糊精浓度、流动相pH值、三乙胺(TEA)、甲醇、柱温、流速等进行了系统研究,建立了羟丙基-β-环糊精流动相添加剂法拆分氯噻酮对映体的方法。使用HarbonLichrospher-C18色谱柱(5μm,150 mm×4.6 mm),流动相为V(甲醇)∶V(水相)=20∶80(水相含30 mmol/L HP--βCD0、.1 mol/L Na2HPO4、体积分数2%的TEA、pH5),流速为0.8 mL/min,室温下拆分,氯噻酮对映体可得到良好分离。
Chlorthalidone enantiomers were separated by reversed phase high performance liquid Chromatography using hydroxypropyl-β-cyclodextrin as a chiral mobile phase additive. The main influence such as concentration of hydroxypropyl-β-cyclodextrin, pH of mobile phase, TEA additive, methanol, temperature of column, flow rate were investigated systematically, and the method of resolution of Chlorthalidone enantiomers using hydroxypropyl-β-cyclodextrin as the chiral mobile phase additive was set up. The results showed that nearly baseline separation was obtained when Harbon Lichrospher-C18 chromatography column(5μm, 150 mm×4.6mm) was used, and the mobile phase was composed of methanol : aqueous phase = 20 : 80 (aqueous phase was composed of 30 mmol/L HP-β-CD, 0. 1 mol/L Na2HPO4, 2%TEA, pH 5), at the flow rate of 0.8 mL/min, being resolved under the ambient temperature.
出处
《分析试验室》
CAS
CSCD
北大核心
2005年第11期22-24,共3页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金资助项目(20376085)
中南大学研究生教育创新工程(040206)
关键词
高效液相色谱
手性流动相添加剂
羟丙基-Β-环糊精
拆分
氯噻酮对映体
High performance liquid chromatography
Chiral mobile phase additive
Hydroxypropyl-β-cyclodextrin
Chlorthalidone enantiomers
Resolution