菊粉酶特性的初步研究

Primary Study on Properties of Inulinase

以菊粉为底物研究了pH值、温度、底物浓度、金属离子对菊粉酶活性的影响及酶活随反应时间的变化情况，并测定该菊粉酶的米氏常数和酶活。实验结果表明，该菊粉酶反应的最适pH值为4.7，最适温度为60℃，Ca^(2+)和Zn^(2+)对菊粉酶有激活作用。该菊粉酶的米氏常数Km为0.35mol／L，V_(max)为0.52mg／min。

The inulinase activities were investigated with inulin as substrate in different pH, temperature, substrate concentration and metal ions, as well as the change of inulinase activities during reactions. The Michaelis constant and activities of the inulinase were also studied in this paper. Our results showed that the optimum pH of inulinase was 4.7, and the optiumum temperature was 60℃, and that Ca^2＋ and Zn^2＋ could improed the activity of in- ulinase. Mcihaelis constants of inulinase were Km = 0.35mol/1 and Vmax = 0.52 mg/min.