COX-2活性变化对HP感染胃癌变中p27^(kipl)蛋白表达的影响

The effect of COX-2 activity change on expression of p27^(kipl) during the course of gastric cancerization(after) HP infection

目的通过研究幽门螺旋杆菌(HP)感染胃癌变过程中不同病理组织类型的环氧化酶2(COX-2)和p27kipl蛋白表达,探讨HP致癌的分子生物学机制。方法经胃镜和病理诊断明确的HP感染患者的不同胃粘膜组织标本共200例。包括慢性浅表性胃炎(CSG)、肠上皮化生(IM)、不典型增生(AH)、胃癌(GCA)各50例,分别以ABC和SP法测定COX-2和p27kipl蛋白表达情况。结果COX-2随癌变过程CSG→IM→AH→GCA的阳性率和表达强度不断提高,分别为12%、42%、52%、74%,GCA组与其它组相比,差异显著(P<0.05)。而p27kipl随癌变过程,CSG-IM-AH-GCA表达率逐步下降,分别为86%、46%、34%和26%,COX-2和p27kipl表达呈显著负相关。结论COX-2是HP感染胃癌变过程的早期事件,并参与了胃癌变的全过程,COX-2抑制了p27kipl的蛋白表达。

Objective To explore the molecular biological mechanism of cancerization in the different gastric pathologic tissue types after helicobacter pylori（HP） infection by studying the expres- sion of cyclooxygenase-2 （COX-2） and p27^kipl. Methods Two hundred samples of gastric mucous membrane were selected, which were taken from the patients definitely infected with HP diagnosed by gastroscope and pathology： chronic superfieal gastritis （CSG）, intestinal metaplasia （IM）, atypical hyperplasia（AH） and gastric carcinoma（GCA） with 50 specimens each. The expressions of COX-2 and p27^kipl were detected by ABC and SP respectively. Results Along with the development of cancerization,positive incidence and expression intensity of COX-2 increased gradually, which were 12%, 42 %, 52 % and 74 % for CSG, IM, AH and GCA respectively. The positive rate of COX-2 expression was significantly higher in group GCA than that in the other three groups（P〈0. 05）. But the change of p27^kipl expression was opposite with the positive rates of 86 %, 46%, 34% and 26% for CSG, IM, AH and GCA respectively. There was a notable negative correlation between the expression of COX-2 and p27^kipl. Conclusion COX-2 is the early event during the course of gastric cancerization after HP infection and takes part in the total process. COX-2 inhibits the expression of p27^kipl.