日本血吸虫成虫38kDa天然分子抗原的分离纯化与免疫保护性的研究

Isolation and purification of the 38 kDa adult worm antigen of Schistosoma japonicum and its immuno protection

目的研究日本血吸虫成虫可溶性抗原38kDa(SjAWA38kDa)的动物保护作用,并对SjAWA38kDa作为抗日本血吸虫病分子疫苗的可行性进行评估。方法用SDS-PAGE电泳、切胶、电洗脱、超滤等方法分离纯化SjAWA38kDa,继而用SjAWA38kDa免疫昆明小鼠,然后进行尾蚴攻击感染(40尾/只),进行减虫率和减卵率研究。结果SjAWA 38kDa天然蛋白质分子能刺激机体产生抗日本血吸虫的作用,其减虫率为33.8%,减卵率为51.7%,有统计学意义(P<0.05)。结论SjAWA 38kDa分子抗原具有刺激机体产生抗日本血吸虫病的免疫保护作用,可以作为抗日本血吸虫病分子疫苗研究的靶标。

To investigate the immunoprotection of the 38 kDa soluble antigen of adult worm of Schistosoma japonicum （SjAWA38kDa） and to evaluate its feasibility of being used as a molecular vaccine antigen, this protein antigen was prepared through SDS-PAGE electrophoresis, protein band resection, electro-elusion and ultrafiltration, and the isolated product of protein was used as antigen to immunize Kunmin strain of mice. After 3 courses of immunization, mice were challenged with cercariae of S. japonicum, and the reduction rates of worms and eggs were compared with those of the control group with inoculation of PBS. It was found that the mono-specific antibody response against SjAWA38kda protein could be induced in mice immunized with this protein. The reduction rates of worms and eggs of the experimental groups of mice were 33.8 % and 51.7 % respectively, with a significant difference. It is concluded that the 38 kDa adult worm antigen of S. japonicum can protect the Kunmin strain of mice from the infection with this parasite, and it can be regarded as the target antigen for the preparation of vaccine against S. japonicum infection.