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蛋白质定向进化的研究技术及应用 被引量：2

The Study and Application on Directed Evolution of Proteins
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摘要 蛋白质定向进化是在人类改造蛋白质的过程中产生的。蛋白质定向进化通常分三步进 行,即随机诱变、体外重组和筛选。每一步都有多种研究技术。蛋白质定向进化大大加速了人类 改造蛋白质的步伐,在实际应用研究和基础理论研究中都具有重要意义。 Directed evolution of proteins occurs in the course of human being＇ s engineering proteins. It is usually based on the combination of random mutagenesis, in vitro recombination and screening. There are many technologies in every step. Directed evolution of proteins greatly accelerates the process of engineering proteins and plays an important role in applied and theoretical study.
作者 谢晚彬 谢和芳
机构地区 西南农业大学
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第B04期16-18,共3页 China Biotechnology
基金 国家自然科学基金资助项目(30270323)
关键词 蛋白质工程 蛋白质定向进化 易错PCR DNA改组技术 筛选 Protein engineering Directed evolution of proteins Error-prone PCR DNA shuffling Screening
分类号 Q51 [生物学—生物化学] Q784 [生物学—分子生物学]
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参考文献19

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  • 2Harayama S. Trends Biotechnology, 1998. 16(2) : 76 - 84.
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二级参考文献18

  • 1[1]Zhang J-H, Dawes G, Stemmer W P C. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc Natl Acad Sci USA, 1997, 94(9):4504～4509
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中国生物工程杂志
2005年 第B04期

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