焊接工人抗6－TG的外周血淋巴细胞突变率的检测

Detection ot 6-thioguanine-reslstant mutant frequency in welders'perlpheral blood lymphocytes

为了探讨焊接烟尘的致突变效应，应用人外周血淋巴细胞抗ＴＧ￣ｒ试验对上海沪东造船厂的３０名焊工进行了检测。结果接触组的突变体数Ｖ＝８．１１×１０￣(－３)±５．１３×１０￣(－３)，显著高于对照组（Ｖ＝３．０７×１０￣(－３)±２．９２×１０￣(－３)），经秩和检验，Ｐ＜０．００１，故焊接烟尘可诱导体内体细胞基因ｈｐｒｔ位点产生突变。另外突变体数随着年龄和吸烟量的增加而增加，且具有统计学意义。

TG￣r mutant frequency in peripheral blood lymphocytes of 30 workers in ShanghaiHudong Ship Yard exposed to welding fume was detected by 6-thioguanine-resistant (TG￣r)assay.Theresults showed that the welders had significantly higher TG￣r mutant frequency in T lymphocytes thancontrol group,the TGr mutant frepuency was also significantly increased with the age and smoking.