一种简单的细胞杂交技术

A Simple Technique of Somatic Cell Hybridization

一种简单的细胞杂交技术宋福津（中国医学科学院，中国协和医科大学，协和医院，北京１００７３０）关键词细胞杂交技术中图号Ｒ３２９．２＋６自１９６４年Ｌｉｔｔｌｅｆｉｅｌｄ创建了用缺少酶的突变体和ＨＡＴ液选择杂种细胞技术以来，许多类似技术相继建立，为基因定...

Abstract Since the first report on the use of mutants lacking enzymes and HAT medium for hybrid selection(Littlefield,1964),enormous similiar techniques,at least,have been basically established.These techniques have opened newavenues to assigning genes,analysing gene regulation in eukaryotic cells and studying mechanisms which govern cell growth and differentiation during normal embryonic development,wound healing and tumor formation,But some problems still remain.For instance,such techniques need about one year to mutate both kinds of cells before fusion and selection,A simple technique of somatic cell hybridization without cell mutation has been developed,and by utilizing different appearances of cells before and after trypsinization,two kinds of cells may be fused and selected in some hours.Up-to-date six kinds of cells have been tested and found successful.Some hundreds of tests have been repeated,and modified,and the analyses by different methods have shown that the technique is both stable and reliable.