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一种简单的细胞杂交技术

A Simple Technique of Somatic Cell Hybridization
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摘要 一种简单的细胞杂交技术宋福津(中国医学科学院,中国协和医科大学,协和医院,北京100730)关键词细胞杂交技术中图号R329.2+6自1964年Littlefield创建了用缺少酶的突变体和HAT液选择杂种细胞技术以来,许多类似技术相继建立,为基因定... Abstract Since the first report on the use of mutants lacking enzymes and HAT medium for hybrid selection(Littlefield,1964),enormous similiar techniques,at least,have been basically established.These techniques have opened newavenues to assigning genes,analysing gene regulation in eukaryotic cells and studying mechanisms which govern cell growth and differentiation during normal embryonic development,wound healing and tumor formation,But some problems still remain.For instance,such techniques need about one year to mutate both kinds of cells before fusion and selection,A simple technique of somatic cell hybridization without cell mutation has been developed,and by utilizing different appearances of cells before and after trypsinization,two kinds of cells may be fused and selected in some hours.Up-to-date six kinds of cells have been tested and found successful.Some hundreds of tests have been repeated,and modified,and the analyses by different methods have shown that the technique is both stable and reliable.
作者 宋福津
机构地区 中国医学科学院
出处 《中国医学科学院学报》 CAS CSCD 北大核心 1996年第4期305-309,共5页 Acta Academiae Medicinae Sinicae
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参考文献4

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