摘要
荧光光谱法和紫外-可见光谱法研究了在模拟人体生理条件下,氨苯砜和牛血清白蛋白结合反应的特征,发现氨苯砜对BSA有较强的荧光猝灭作用,且氨苯砜的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠,获得其作用距离和结合过程的基本热力学参数。
The binding characteristics of dapsone (DS) and bovine serum albumin (BSA) were studied by fluorescence and absorption spectroscopy in aqueous solution. DS had a powerful ability to quench the BSA fluorescence via non-radiation energy transfer mechanism. The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal equation, from which the reaction distance and the thermodynamic parameters were obtained.
出处
《光谱实验室》
CAS
CSCD
2005年第6期1192-1195,共4页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金资助项目(20275028)
关键词
氨苯砜
牛血清白蛋白
荧光光谱法
吸收光谱法
热力学
Dapsone, Bovine Serum Albumin, Fluorescence Spectrometry, Absorption Spectrometry, Thermodynamics.