抗人膀胱癌VH－PE在大肠杆菌的表达及初步鉴定

An immunotoxin using VH-PE for urinary bladder cancer

为了减少异源抗体免疫原性和增强其抗体细胞毒效应，利用基因工程抗体技术将已获得的具有与人膀胱癌细胞结合活性的单抗重链可变区基因（ＶＨ）亚克隆至构建好的含有重组绿脓杆菌外毒素基因（ＰＥ）的表达载体中，经大肠杆菌表达，研究制备具有导向治疗作用的重组免疫毒素。经间接免疫荧光试验等技术初步鉴定，所获得的免疫毒素与人膀胱癌细胞具有一定的结合效应。

For targeting therapy, genetic engineering antibodies would be preferred to mouse McAbs as the foreign immunogobulin might ilicit an anti-globulin response and hypersensitivity. Functional antibody genes coming from a hybridoma antibody that reacted with human urinary bladder cancer cells have been cloned into an E. Coli expressive vector containing a portion of pseudomonas exotoxin gene. The latter could produce a cytotoxin protein. The results revealed that the expressed protein could block the binding of parent McAb with urinary bladder cancer cells so as to kill the malignant cells Although the blocking activity was not so strong, it might be possible to select a better and more suitable targeting product in the future.