前列腺癌组织中细胞外基质的免疫组织化学研究

Immunohistochemical studies of extracellular matrix in prostatic carcinoma tissues

为探讨前列腺癌（ＰＣａ）细胞的转移机制，应用免疫组织化学ＡＢＣ法在良性前列腺增生（ＢＰＨ）及ＰＣａ组织中对基膜连接蛋白（ＬＮ），胶原Ⅳ型蛋白（ＣＯＬⅣ），纤维连接蛋白（ＦＮ）等细胞外基质（ＥＣＭ）蛋白的表达进行检测，结果ＢＰＨ组织中基底膜部位ＬＮ，ＣＯＬⅣ及ＦＮ的染色呈连续线状分布，ＰＣａ组组织基底膜部位ＬＮ，ＣＯＬⅣ及ＦＮ的阳性染色呈碎片状或断线状分布。ＣＯＬⅣ在ＢＰＨ与ＰＣａ组织中的强阳性率无显著性差异（Ｐ＞０．０５）。结果提示ＥＣＭ在ＰＣａ组织中基底膜部位的明显缺失（非连续线状分布）可能是肿瘤转移的重要因素。

Immunohistochemical studies of the distribution of laminin(LN),type Ⅳcollagen(COL Ⅳ)and fibronectin(FN)in benign hyperplastic prostate tissue and prostatic carcinoma tissue were carried out.In benign prostatic hyperplasis,LN, COL Ⅳ and FN diplayed a continuous linear distribution in the basement membrane while these appeared scattered and interrupted in prcatatic carcinoma tissue and the rate of strong immunostaining being not enhanced(P>0.05),It was postulated that in prostatic carcinoma, a decrease of extracellular matrix in the basement membrane might play an important role in cancer metastasis.