前列腺癌增殖细胞核抗原的免疫组化研究

Expression of proliferating cell nuclear antigen in human prostatic cancer

为了探讨增殖细胞核抗原（ＰＣＮＡ）与前列腺癌分化程度的关系及其对预后的影响，采用ＰＣＮＡ单克隆抗体及免疫组织化学链菌素－生物素标记法（ＬＳＡＢ法），对３８例前列腺癌穿刺的石蜡标本中ＰＣＮＡ的表达进行了检测，以１５例前列腺良性增生及１０例正常前列腺组织作为对照，通过观察、统计组织切片中ＰＣＮＡ表达阳性细胞数目，计算出ＰＣＮＡ表达的阳性指数。结果发现，在低分化癌、中分化癌、高分化癌、前列腺良性增生及正常前列腺组织中，其ＰＣＮＡ阳性指数依次为８．０±４．０％、５．２±１．８％、２．４±１．１％、１．１±０．３％、０．８±０．６％，呈逐渐降低趋势。３８例前列腺癌患者经去势手术后随访１年以上者２５例，其中死亡６例，死亡组ＰＣＮＡ表达平均指数为９．０±３．０％，非死亡组为５．０±４．２％。两组比较Ｐ＜０．０５。结果表明：ＰＣＮＡ是判断细胞增殖程度的重要指标，对估计前列腺癌的分化程度及推测预后具有重要参考价值。

Expression of proliferating cell nuclear antigen( PCNA)was detected in prostatic carcinoma tissue obtained from needle biopsy for 38 cases by means of specific mouse monoclonal antibody and immunohistochemical technique(LSAB method).15 benign hyperplastic prostate tissues and 10 normal prostate tissues served as controls. The average PCNA labeling index of the poorly differentiated tumors was 8.0±4.0%, the moderately differentiated tumors 5.2±1.8%, the well differentiated tumors 2.4±1.1%, the BPH tissues 1.1±0. 3%and the normal prostate tissues 0.8±0. 6%, showing gradual decline in the above order. Castration has been undertaken in all and 25 patients have been followed up for more than one year, 6 of them already died and the average PCNA labeling index of their carcinoma tissues was 9.0±3.0%and that of the other 19 patients 5.0±4. 2%(P<0. 05). PCNA expression was believed to be related to tumor differentiation and might be of value in the assessment of the prognosis of prostatic adenocarcinoma.