摘要
为了探讨先天性胆道闭锁(CBA)的原因,分析肝脏病理变化、临床预后与巨细胞病毒(CMV)感染的关系。取14例CBA患儿术中肝脏及肝门淋巴结标本,一份进行多聚酶链反应巨细胞病毒基因(PCR-CMV-DNA)检测,另一份肝脏标本做HE染色光镜检查。结果:14例中CMV阳性占8例。肝脏病理检查表明随手术时日龄增加,胆管增生明显。比较CMV阳性组与CMV阴性组肝细胞病变、淤胆无差异,肝纤维化CMV阴性组重于阳性组;肝细胞坏死CMV阳性组重于阴性组。随访8~20个月,生存6例,其中CMV阳性5例,死亡8例。CMV感染可能是CBA的病因之一,肝纤维化程度、术后胆管炎发生的频率和程度与预后关系密切。从临床归转看CMV感染阳性组预后较好。
In order to investigate the etiology . pathologic changes of congenital biliary atresia (CBA) and the relations of its prognosis with the infection of cytomegalovirus (CMV ) , 14 cases with CBA including those associated with CMV hepatitis are studied. The liver tissues and lymph nodes of porta hepatis obtained through operation were -both detected of CMV by polytnerase chain reaction (PCR-CMV-1DNA ) and examined microscopically with HE stain. Eight being PCR-CMV- 1DNA positive meant the incidence of CMV infection was 57. 1% . The pathological changes in CBA and the degree of hyperplasia of bile ducts correlated with the increase of patients, age. There was no significant difference in cholestasis , liver cell necrosis , and giant cell changes between CMV positive group and negative group. Liver fibrosis was obviously remarkable in CMV negative group , but the necrosis of liver was severe in CMV positive group. On following-up of 8-20 months . 5/6 cases survived in CMV positive group. Conclusion , Some of CMA might be a causative factor and. give rise to a better prognosis of CBA.
出处
《中华小儿外科杂志》
CSCD
1996年第5期273-275,共3页
Chinese Journal of Pediatric Surgery
关键词
胆道闭锁
聚合酶链反应
巨细胞病毒
肝内胆管
Biliary tract : Polymerase chain reaction
Cytomegaloviruses
Bile ducts , intrahepatic