颈交感神经干离断对妊娠高血压综合征大鼠NOS活性及雌激素含量的影响

Influence of transection of cervical sympathetic trunck on activity of NOS and content of estrin in rats with pregnancy-induced hypertension syndrome

目的观察颈交感神经干离断(TCST)对妊高征(PIH)大鼠一氧化氮合酶(NOS)活性及雌激素含量的影响。方法妊娠大鼠随机分5组,每组15只,对照组(A):自妊娠14d开始皮下注射生理盐水至孕20d;妊高征1组、2组(B1、B2):自妊娠14d开始皮下注射L-NAME分别为12.5mg/100g/d和6.25mg/100g/d,至孕20d;手术组(C):妊第14天行右TCST,余同B1;假手术组(D):妊第14天行右颈交感神经干分离,但不离断,余同B1。孕21天剖宫取仔。观察孕鼠血压、尿蛋白,胎鼠的大小、体重、畸形率、死亡率、孕鼠血清NOS活性及游离雌三醇(FE3)含量。结果血压、尿蛋白除基础值外B1、B2组各时点值明显高于A组(P<0.01);B2组明显低于B1组(P<0.01);C组与A相比差异无显著性(P>0.05)。胎鼠体重、大小B1、B2组明显低于A组(P<0.01);B1组明显低于B2组(P<0.05,0.01);C组与A相比差异无显著性(P>0.05)。死亡率、畸形率变化与上述指标相反。孕鼠血清NOS活性和FE3含量B1、B2组明显低于A组(P<0.01);B1组明显低于B2组(P<0.01,0.05);C组明显高于B1组(P<0.01),与A组比差异无显著性(P>0.05)。结论TCST可保护孕鼠免受PIH的影响,可能与其具有提高孕鼠NOS活性和增加雌激素含量有关。

[Objective] To observe the influence of transection of the cervical sympathetic track （TCST） on the activity of NOS and the content of estrin in the rats with pregnancy-induced hypertension syndrome （PIH）. [Methods] Pregnant Wistar rats were randomly divided into 5 groups. Control group （A）： Saline was injected subcutaneously from the 14th day to 20th day of gestation. PIH groupl （B1） and group2 （B2）： L-NAME was respectively injected with 12.5 rag/100 g and 6.25 rag/100 g every day, then the other procedures were the same as group A. Operation group（C）： TCST was operated on the 14th day of the gestation, then the other procedures were the same as group B1. Sham operation group （D）： The cervical sympathetic trunk was only separated and exposed on the 14th day of the gestation, then the other procedures were the same as group B1. The changes of pregnant rats and their pups were observed. The NOS activity and the content of estrin in the blood serum of pregnant rats were measured. [Results] Except base value of the BP and protein in urine of the pregnant rats, they in Group B1, B2 were higher than those in Group A significantly （P 〈0.01）, and in Group B2 were lower than those in Group B1 markedly （P 〈0.01）. There was no marked difference between GroupC and Group A（P 〉0.05）. In comparrsion with those in Group A, the size and body weigh of fetus in Group B1, B2 decreased markedly （P 〈0.01）. The above indexes in Group B1 were lower than those in Group B2 markedly （P 〈0.01, 0.05）. The changes of the rate of embryo absorption and fetal death, and deformity rate of the fetal rats were contrary to the above indexes. Significant difference was not found between GroupC and Group A （P 〉0.05）. The NOS activity and the content of estrin in the blood serum in Group B1, B2 were lower than those in Group A markedly （P 〈0.01）. Those in GroupB1 were lower than those in Group B2 obviously （P 〈0.01,0.05）. Those in Group C were higher than those in Group B1 markedly （P 〈0.01）, and there was no significant difference compared to Group A （P 〉0.05）. [Conclusion] TCST make pregnant rats avert the effect of PIH, and it can be related to the improving of the NOS activity and the content of estrin in the blood serum.