Ets-1、c-myc在骨巨细胞瘤中的表达及意义

Expression and significance of Ets-1,c-myc in giant cell tumor of bone

目的探讨Ets-1、c-myc在骨巨细胞瘤中的表达与肿瘤预后复发的关系。方法利用免疫组化SP法检测Ets-1、c-myc在30例采用刮除手术治疗的骨巨细胞瘤患者石蜡切片中的表达。结果30例骨巨细胞瘤患者,其中复发10例,复发率33.3%。Ets-1在复发组中阳性表达5例,未复发组4例,差异无显著性。复发组与未复发组c-myc阳性表达分别为6例、4例,存在统计学差异。两者同时表达在复发组与未复发组分别为4例、1例,Ets-1和c-myc同时过表达与骨巨细胞瘤复发有关。结论在排除手术方式影响下,c-myc表达与Ets-1、c-myc同时表达可以作为帮助评估骨巨细胞瘤预后复发的参考指标。

[Objective] To analyze the expression of Ets-1 and c-myc in giant cell tumor of bone which were surgically treated by local excision. [Methods] The expression of Ets-1 and c-myc was examined in 30 giant cell tumor of bone specimens which were formalin-fixed and paraffin-embedded sections by streptavidin-biotin immunoperoxi-dase method. [Results] There were 10 recurrent cases in this 30 patients group treated by local excision. 5 of 10 recurrent cases of giant cell tumor of bone showed an Ets-1 positive reaction. 4 of 20 primary cases of giant cell tumor of bone showed positive reaction. Ets-1 expression significantly didn,t increase （P 〉0.05） in recurrent group. 6 of 10 recurrent cases of giant cell tumor of bone showed an c-myc positive reaction,and 4 of 20 primary cases of giant cell tumor of bone an c-myc positive reaction, c-myc expression significantly increased （P 〈0.05） in recurrent group. 4 of l0 recurrent cases of giant cell tumor of bone showed Ets-1 and c-myc positive reaction, and 1 of 20 primary cases of giant cell tumor of bone showed Ets-1 and c-myc positive reaction. There was a staffstically significant co-rrelation between the overexpression of Ets-1 and c-myc in giant cell tumor of bone and recurrence （P 〈0.05）. [Conclusion] Expression of c-myc and expression of both Ets-1 and c-mye can help estimate the prognosis and recurrence of giant cell tumor of bone treated by local excision.