高效液相色谱-质谱法测定猪组织中硝基咪唑类药物残留量

Determination of nitroimidazole residues in swine tissues by LC-MS

建立了一种高灵敏度和高选择性的检测猪组织中硝基咪唑类药物残留的高效液相色谱-大气压化学电离质谱(APCI)法,可同时进行猪组织中3种硝基咪唑类药物残留的确证分析.本方法检测猪肌肉、肝脏、肾脏和皮下组织中硝基咪唑类药物的检测限为0.5～1μg/kg;空白组织添加硝基咪唑类药物水平为0.5、1、2或4μg/kg时,测得回收率:肌肉为61.1%～85.4%,肝脏为62.3%～80.4%,肾脏为61.6%～82.0%,皮下组织为63.6%～84.1%.

A sensitive and selective method of high performance liquid chromatography with atmospheric pressure chemical ionization （APCI） mass spectrometry for the simultaneous detection of ronidazole（ RNZ）, metronidazole （MNZ） and dimetridazole（DMZ） in swine tissues has been developed. The limits of detection were 0.5 -1 μg/kg for MNZ, RNZ and DMZ in muscle, fat, kidney and liver. Tissues fortified 3 kinds of drugs at 0.5, 1, 2 and/or 4 μg/kg, averge recoveries ranged from 61.1% to 85.4% in muscle, from 62.3% to 80.4% in liver, from 61.6% to 82.0% in kidney and from 63.6% to 84.1% in fat.