肺出血新生儿肺泡灌洗液中肺表面活性蛋白A的变化及意义

Change and significance of surfactant protein A in BALF of newborns with pulmonary hemorrhage

目的研究肺表面活性蛋白A(SP-A)在肺出血新生儿支气管肺泡灌洗液(BALF)中的变化及其与预后的关系。方法用斑点免疫印迹法分别测定对照组(n=15),肺出血存活极期组(存活组,n=14),肺出血恢复期组(恢复组,n=14)和肺出血死亡组(死亡组,n=6)新生儿BALF中SP-A水平。结果肺出血组BALF中SP-A含量较对照组显著降低(P<0.01);死亡组SP-A降低更明显,明显低于存活极期组(P<0.05);肺出血恢复期,SP-A水平明显恢复,接近对照组(P>0.05)。结论肺出血新生儿BALF中SP-A降低,与疾病严重程度密切相关,可作为肺出血高危新生儿预警和预后指标。

Objective To study the change of surfactant protein A（SP-A）in bronchoalveolar lavage fluid （BALF） and the relationship with prognosis in newborns with pulmonary hemorrhage. Methods SP-A was measured by Western-dot blot analysis in group control（ n = 15）, group survival with pulmonary hemorrhage within an acute phage（survival, n = 14 ）, group survival with pulmonary, hemorrhage within a recover phage （recovery, n = 14）and group death （death, n = 6）. Results The content of SP-A in BALF in newborns with pulmonary hemorrhage was significantly lower than that in group control （P 〈 0.01 ）. The level of SP-A in BALF in group death was found significantly lower than that in group survival（P 〈 0.05）. The level of SP-A in group recovery elevated to approach that in group control（P 〉0.05）. Conclusion The content of SP-A in BALF may be considered as an indicator of the predict and the prognosis of pulmonary hemorrhage in new- borns.