摘要
研究了玉米蛋白粉中玉米黄素的抗肿瘤活性。采用体外培养的人癌细胞株———人口腔鳞癌KB细胞作为对象,观察玉米黄素对抑瘤活性、细胞周期的影响.发现玉米黄素对KB细胞24 h的半数抑制浓度IC50为35μmol/L,在20μmol/L时即明显抑制KB细胞的对数生长;细胞周期分析显示时相左移,G1期细胞百分率增多,S期、G2+M期百分率降低.DNA凝胶电泳显示30μmol/L玉米黄素作用72 h能使KB细胞的DNA发生明显的降解.因此从玉米蛋白粉中提取的玉米黄素能明显抑制KB细胞生长,其抗肿瘤活性强,对细胞周期的影响提示G0期阻滞.
AIM: To determine the antitumor effects of zeaxanthin from corn gluten meal against human oral squamous carcinoma KB cellline in vitro. METHODS: Antitumor activity was measured using IC50, cell growth curve and the cell cycle were analyzed by flowcytometric (FCM) technique and DNA electrophoresis. RESULTS:IC50 of zeaxanthin was 35μmol/L on KB celline when incubation for 24h. Zeaxanthin obviously inhibited the growth curve of KB cell at 20 μmol/L, in the tumor ceils of treated group. FCM analysis revealed that zeaxanthin KB cells percentage of G2/M phase, the S phase, and G1 phase decreased, but percentage of G: + Go phase increased. The tests of Agarose gel electrophoresis of DNA from cells treated with zeaxanthin revealed "DNA ladder". CONCLUSION: Zeaxanthin could remarkably inhibited KB cellline proliferation and showed a high antitumor activity. The effect of zeaxanthin on cell cycle might be the one of the important reasons for inhibiting carcinoma cell growth.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2005年第6期34-37,共4页
Journal of Food Science and Biotechnology
基金
国家"863"计划项目(2001AA248011-01)资助课题