玉米蛋白粉中玉米黄素对人口腔鳞癌细胞株KB增殖的抑制

Inhibitory Effects of Zeaxanthin from Corn Gluten Meal on Human Oral Squamour Cell Carcinoma

研究了玉米蛋白粉中玉米黄素的抗肿瘤活性。采用体外培养的人癌细胞株———人口腔鳞癌KB细胞作为对象,观察玉米黄素对抑瘤活性、细胞周期的影响.发现玉米黄素对KB细胞24 h的半数抑制浓度IC50为35μmol/L,在20μmol/L时即明显抑制KB细胞的对数生长;细胞周期分析显示时相左移,G1期细胞百分率增多,S期、G2+M期百分率降低.DNA凝胶电泳显示30μmol/L玉米黄素作用72 h能使KB细胞的DNA发生明显的降解.因此从玉米蛋白粉中提取的玉米黄素能明显抑制KB细胞生长,其抗肿瘤活性强,对细胞周期的影响提示G0期阻滞.

AIM： To determine the antitumor effects of zeaxanthin from corn gluten meal against human oral squamous carcinoma KB cellline in vitro. METHODS： Antitumor activity was measured using IC50, cell growth curve and the cell cycle were analyzed by flowcytometric （FCM） technique and DNA electrophoresis. RESULTS：IC50 of zeaxanthin was 35μmol/L on KB celline when incubation for 24h. Zeaxanthin obviously inhibited the growth curve of KB cell at 20 μmol/L, in the tumor ceils of treated group. FCM analysis revealed that zeaxanthin KB cells percentage of G2/M phase, the S phase, and G1 phase decreased, but percentage of G： ＋ Go phase increased. The tests of Agarose gel electrophoresis of DNA from cells treated with zeaxanthin revealed ＂DNA ladder＂. CONCLUSION： Zeaxanthin could remarkably inhibited KB cellline proliferation and showed a high antitumor activity. The effect of zeaxanthin on cell cycle might be the one of the important reasons for inhibiting carcinoma cell growth.