Identification of seeds of Pinus species by microsatellite markers

276 对教材(原子并且叶绿体微卫星) 从七种发展了松科为跨种类的可转移性被选择并且识别到 tenPinus 种类(P。massoniana， P。kesiya， P。tabulaeformis， P。densiflora， P。thunbergii， P.caribaea， P。taeda， P。yunnanensis， P。densata， P。sylvestris ) 属于派别。Pinusby BSA (bulked 分离分析) 方法。结果显示出 276 中的那 23 个(8.0%) 标记是成功的在十种有扩大产品，并且(21.7%) 23 中的 5 个是多态的生气种类和缺乏在种类以内多态。10 Pinus 种类中的八个被使用单个教材识别，二并且教材的更多的联合，但是仍然为识别另外的 2 种类没有有效 SSR 教材(P。kesiya 和 P。densata ) 。

The 276 pair-primers （nuclear and chloroplast microsatellite） developed from seven species of Pinaceae were selected and identified for cross-species transferability to ten Pinus species （P massoniana, P kesiya, P tabulaeformis P densiflora, P thunbergii, P caribaea, P taeda, P yunnanensis, P densata, P sylvestris）belonging to Sect. Pinus by BSA （bulked segregate analysis） method. The results showed that 23 of 276 （8.0%） markers were successful to have amplification product in ten species, and 5 of 23 （21.7%） were polymorphic cross species and lack of polymorphic within species. Eight of 10 Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species （P. kesiya and P. densata）.