摘要
本实验室已发现，在正常生理状态下脑室管膜某些区域的伸长细胞呈Ｐ物质免疫反应阳性。本实验采用同位素示踪放射自显方法观察室管膜伸长能否特异性吸收和转运脑室注射的＾３Ｈ－ＳＰ，以进一步探讨这些ＳＰ免疫反应阳性伸长细胞中的ＳＰ是否为外源的。
We had observed that Substance P (SP) immunoreactive tanycytes situated in some areas of the ependyma. The isotopic tracer technique & autoradiography were applied to study whether the tanycytes can especially absorb and transport 3H - SP injected into the third ventricle. The results were as followed: There were lots of 3H - SP grains in the ependymocytes, except for those in the central canal of medulla oblongata. 3H - SP grains also existed in the junctions of these ependymocytes. There was no difference in theammount of 3H -SP grains among these ependymocytes. When the ependymocytes' skeletons were destroyed and the transportation of 3H - SP was hindered, the 3H - SP grains accumulated on the surface of the ependymocytes, inside these cells and in the subependyma cells. These results suggested that the ependymocytes could absorb 3H -SP from the CSF and then transport them to the brain actively, and there was no difference in the abilities of absorption and transportation of 3H-SP between the tanycytes and common ependymocytes. Therefore, it was concluded that the tanycytes could not especially absorb and transport the 3H-SP injected into the third ventricle, and the SP in these immunoreactive tanycytes might not be taken in from the CSF. The results have provided direct evidence to study further the tanycytes' physiological functions, such as synthesis of neuropeptides, and the important roles in the brain - cerebrospinal fluid circuits.
