海棠果插穗的内源激素水平及其与扦插生根的关系

Endogenous Hormones Levels in Cuttings of Malus prunifolia and Their Relations to Rooting

海棠果(Malus prunifolia)扦插繁殖的试验结果表明,嫩枝扦插效果好于硬枝扦插,并且母树年龄是影响其扦插生根的重要因子。母树年龄为2年生时,生根率达95%以上。本文在此试验的基础上,对嫩枝和硬枝插穗中内源植物激素水平进行了测定。结果发现嫩枝插穗中IAA、CTKs的含量要高于硬枝插穗中IAA、CTKs的含量,并且随母树年龄的增加而降低;相反,嫩枝插穗中ABA含量要低于硬枝插穗中ABA的含量,并且随着母树年龄的增加而增加。实验结果表明:IAA、ABA的含量和扦插生根有密切关系。IAA促进生根,ABA抑制生根。IAA/ABA比值可以用来衡量海棠果的生根能力。

The experiment results of the cuttage propagation of Malus prunifolia have shown that shoot cuttings root better than hardwood cuttings, and seed - tree age is an important factor which affect the rooting . Seed - tree of two, the rate of rooting got more than 95%. On the basis of these experiments, this paper determined the contents of endogenous plant hormones levels in hardwood and shoot cuttings. It is shown that the contents of IAA, CTKs in shoot cuttings are higher than that in hardwood cuttings, and reduce with raising the age of seed - tree ; on the other hand , the content of ABA in shoot cuttings is lower than that in hardwood cuttings, and increase with raising the age of seed tree. The results demonstrate that the contents of IAA, ABA are closely related to rooting of Malus prunifolia, IAA promotes rooting and ABA inhibits rooting. The experiments point out that IAA：ABA ratio maybe taken for a standard to express the capability of rooting of Malus prunifolia.