摘要
1993年4~5月.采用实验现场结合组织化学技术,观察了水淹对钉螺春季繁殖器螺卵组织化学的影响。结果表明,对照组螺卵卵胚内的SDH、LDH及CCo等与细胞能量代谢有关的酶呈强阳性反应.实验组螺卵卵胚内的SDH.CCo,水淹第10d时降为的阳性反应(+),30d时消失,LDH含量在水淹在20d时未见变化,30d时降为阳性反应(++),40d时消失。水淹30d的螺卵石蜡包理切片,作糖原(PAS)反应,由强阳性变为弱阳性(+).综合上述结果,分析了水淹影响螺卵发育的机制。
The histochemical changes of snail eggs were observed by means of experimental field study method combinatied with histochemical techniques during April-May in 1993.The results showed that the enzymes related with energy metabolism in the embryo of the snail eggs of control group,such as SDH,LDH and CCO etc,appeared as strong positive reaction(++ +).Of the experimental group,the SDH and CCO became mild(+)at the 10th day after flooding and disappeared at the 30th day.And the LDH had the change at the 20th day,positive reaction(++)at the 30th day,no-reaction at the 40th day-PAS reaction of glycogen changed from strong(+++)to mild positive reaction(+)with paraffin wax wrapping and slicing method at the 30th day.The mechanism of impacts on the development of snail eggs by flooding was analyzed and discussed with the above results.
出处
《实用预防医学》
CAS
1996年第3期147-148,共2页
Practical Preventive Medicine
关键词
灭钉螺
水淹
钉螺卵
组织化学
flooding oncomelania snail egg histochemistry.