皮肤低温损伤机制中海藻糖的抗氧自由基作用

Trehalose's protective effects of cryopreserved skin to resist the damage of oxygeon free radical

目的:观察海藻糖在低温储存皮肤组织中的抗氧自由基损伤作用。方法:取新鲜皮肤标本做正常对照组,另行海藻糖和二甲基亚砜处理后液氮低温储存,复温后测定皮肤组织匀浆液中超氧化物歧化酶(SOD)活力,过氧化脂质(LPO)代谢产物丙二醛(MDA)含量。结果:经海藻糖处理后,低温储存的皮肤组织中SOD活力升高,其MDA含量下降。结论:海藻糖是一种有效的稳定剂,在皮肤低温损伤中具有抗氧自由基损伤作用。

Objective： To investigate the protective effects of trehalse during cryoinjury. Methods： Evaluate the level of superoxide dismutaes （SOD） and the amount of malonyldialdehyde（MDA） from the cryopreserved skin. Results：Comparing to the norreal human skin, trehalose could enhance the vitality of SOD and decrease the production of MDA when the skin was frozen. Conclusion： It showed that trehalose may promote the ability on elimination of oxygen free radical and decrease the attack of the oxygeon free radical during cryoinjury.