一种新型兔骺板部分早闭模型的建立

A rabbit model of partial growth plate closure

目的:建立周围性骺板部分早闭兔模型.方法:采用手术方法,将20只4～6周新西兰白兔右侧股骨外髁部的骺板部分挖除,其面积约为股骨远端骺板总面积的40%～45%.左侧仅切开至骨膜作为自身对照.术后通过X线片、大体标本、组织学等手段观察本方法是否可以造成股骨远端的骨桥以及股骨的畸形情况.结果:手术后4月,通过X线观察,左侧股骨平均长度明显长于右侧,外翻角度明显小于右侧,两组之间差异显著.股骨远端生长长度能更加精确的反映股骨远端骺板的生长情况,结果左侧平均生长2.27cm,约占整个股骨生长的57.6%,右侧平均生长0.45cm,只相当于整个股骨生长的21.4%.结论:本方法建立的模型操作简单、成功率高、应用灵活,对于研究儿童骺板部分早闭的发生机制、病理变化及治疗过程中填充物的选择有一定意义.

Objective： To develop the rabbit model of peripheral partial growth plate closure. Methods： There are 20 white New-Zealand rabbit with 4-6 weeks old and weighting 1-1.5kg was used as the experimental animals. We resected part of the growth plate on the fight lateral condyle on the distal femur; the area is about 45%-50% of the total growth plate. The left side femur was served as the sham operation self-control group. After operation we observed if the osseous bridge will appear and the degree of deformity through gross specimen, radiography and histological methods. Resdts： 4 months after operation, the femoral length of left were significantly longer than the fight, and the angle of the valgus deformity of the left side were significantly smaller than the fight side. There is statistically significant difference between the two groups. Conclusion： This is a simple method to make the model of peripheral partial growth plate closure, and the rate of success is very high.