^(131)I标记血卟啉衍生物及其在膀胱癌放射显象中的应用

131 I Labelled Hematoporphyrin Derivative and Its Application in Radioimaging of Bladder Tumors

为了解血卟啉衍生物（ＨｐＤ）在体内的分布情况，利用放射性核素１３１Ｉ标记ＨｐＤ，制备成１３１Ｉ－ＨｐＤ，将其静脉注入带有膀胱移行细胞癌的裸小鼠体内，显示１３１Ｉ－ＨｐＤ在裸小鼠体内相对稳定，并随着时间延长，肿瘤组织中相对放射性强度逐渐增高。对６例膀胱癌患者静脉注射１３１Ｉ－ＨｐＤ后２４、４８、７２小时膀胱区γ闪烁显象，其中５例表现为放射性浓聚区，其位置与手术中所见肿瘤位置相同；１例显象阴性，术中见散在的小肿瘤。说明ＨｐＤ对肿瘤定位、导向治疗有一定意义。

Hematoporphyrin Derivative (HpD) was labbelled with radioactive isotope 131 I, the efficiency of labbelling reached 95%. Identical absorption spectra were confirmed between standard HpD and 131 I HpD ranging from 240 to 800nm. 18 nude mice with transplanted bladder tumor were killed and the tissue levels of HpD and relative radioactivity at 24、48、72 hours after tail venous administration of 131 I HpD were measured. The results indicated that the relative radioactive density of tumor against muscle gradually increased, and its rate was higher than the heart, lung, bladder, but lower than the liver, spleen and kidney at the same period. In order to study the in vivo distribution of HpD in human bladder tumors using gamma scintigraphy, we carried out an 131 I HpD radioimaging study in 6 patients with bladder tumor, 5 of which showed positive images in the bladder and the location of positive images accorded well with the sites of malignant lesions revealed at surgical operation. In one case with negative imaging, small scattering lesions were found at surgical operation.