胰岛素干预加重海马注射AΒ阿尔茨海默病大鼠模型认知损害(英文)

Insulin aggravates amyloid deposition and cognition impairment in an AD-like rat model

目的观察胰岛素对AD样模型大鼠认知、病理、生化指标的影响。方法采用立体定向双侧海马CA3 区注射AΒ42建立AD大鼠模型,皮下注射胰岛素作为干预,观察大鼠MORRIS水迷宫逃避潜伏期、穿越平台欢数, 以及采用免疫组化和ELISA方法,比较脑内AΒ沉积的改变。结果 AΒ+胰岛素组与AΒ组相比,具有较多的海马AΒ沉积(增加35．7％),和更高的AΒ含量(增加23．6％),同时其逃避潜伏期明显延长,穿越平台次数显著减少。结论胰岛素加重AD样模型大鼠的AΒ沉积和认知损害。

Objective to evaluate the impact of insulin 32 male Wistar rats were divided into 4 groups ： Aβ, Aβ ＋ on pathology and cognition in AD-like rat model. Methods insulin, insulin and normal control group. Each group ineluded 8 subjects. Aggregated Aβ solution was injected in CA3 area of bilateral hippocampi, insulin was administered subcutaneously pre-surgery once a day for 7 consecutive days. Behavioral performance in Morris water maze was observed and level of Aβ deposition was assessed by immunohistochemistry staining and ELISA assay. Results Aβ ＋ insulin group had a much higher Aβ level （increased by 23.6% ） and more severe Aβ deposition （ increased by 35.7% ） than the other three groups. And poorer performance in Morris water maze was observed. Conclusion Insulin can aggravate Aβ deposition in the hippocampus of AD-like rat model and worsen its memory loss.