基因工程干扰素γ对小鼠Lewis肺癌转移及脾细胞增殖的影响

EFFECTS OF RECOMBINANT INTERFERON-γON LUNG METASTASIS OF LEWIS LUNG CARCINOMA AND PROLIFERATION OF SPLENOCYTES IN MICE

Ｃ（５７）ＢＬ／６Ｎ小鼠尾静脉注射Ｌｅｗｉｓ肺癌细胞１×１０５后，腹腔注射基因工程干扰素γ（ｒＩＦＮ－γ）１万、５万、２０万ｕ·ｋｇ（－１），连续１０ｄ；小鼠肺湿得减少０．６％、１５．３％、２０．１％；肺转移结节数减少６．６％、３６．７％、５５．０％；ＣｏｎＡ诱导的脾细胞ＤＮＡ掺入量增加３．０％、２５．７％、５２．５％。小鼠环磷酰胺５０ｍｇ·ｋｇ（－１）ｉｐ３ｄ后，尾静脉注射瘤细胞，实验处理同上，小鼠肺湿重减少５．３％、１８．１％、２７．６％；肺转移结节数减少１６．３％、４５．６％、６９．６％；脾细胞ＤＮＡ掺入量增加１７．１％、７２．４％、９０．９％。本文结果表明，ｒＩＦＮ－γ可抑制小鼠Ｌｅｗｉｓ肺癌肺转移，促进ＣｏｎＡ刺激的脾细胞增殖，并与剂量呈正相关。对环磷酰胺处理后免疫功能受抑小鼠的作用更为明显。

Recombinant interferon-γ(rIFN-)inhibited the lung metastasis of Lewis lung carcinomaand increased the proliferation of splcnocytes in mice Ip 1～20×104u· kg(-1) for 10 d,the lung weight ofmice decreased by 0.6%～20.1%; the numbers of metastatic foci decreased by 6.6%～55.0%; and[3H]TdR incorporated radioactivity increased by 3.0%～52.5%.rIFN-γ had a more powerful effects ofanti-metastasis and increasing proliferation of splenocytes in the mice administrated previously withcyclophosphamide than in the unadministrated mice.