摘要
目的研究大鼠心肌梗死后转化生长因子β1(TGF-β1)与Ⅰ型胶原表达。方法结扎左冠状动脉建立大鼠心肌梗死模型,采用免疫荧光染色的方法,通过激光扫描共聚焦显微镜观察TGF-β1与Ⅰ型胶原表达的变化。结果心梗24h后心肌TGF-β1与Ⅰ型胶原表达与假手术组相比明显增强。结论心梗后心肌通过增加TGF-β1、Ⅰ型胶原的表达参与心室重构。
Objective To investigate the expression of transforming growth factor-β1 （TGF-β1） and type Ⅰ collagen after myocardial infarction. Methods Rat model of myocardial infarction was established by ligation of left main coronary artery. Immunofluorescence staining was used to observe the change of transforming growth factor-β1 （TGF-β1） and type Ⅰ collagen with laser scan confocal microscope. Results The expression of transforming growth factor-β1 （TGF-β1） and type Ⅰ collagen in myocardium after 24 h of myocardial infarction were significantly higher than those in sham group at corresponding time. Conclusion It participates in ventricles reconstitution from upregulation of the expression of transforming growth factor-β1 （TGF-β1） and type Ⅰ collagen in myocardium after myocardial infarction.
出处
《中国实验诊断学》
2005年第6期918-920,共3页
Chinese Journal of Laboratory Diagnosis
关键词
心肌梗死
转化生长因子Β1
Ⅰ型胶原
myocardial infarction
transforming growth factor-β1
type Ⅰ collagen
