玉米转录因子zmCBF1的凝胶阻滞分析

Analysis of Maize Transcription Factor zmCBF1 by Gel Retardation Assay

凝胶阻滞试验是分析核酸与蛋白质相互作用的有效方法,在转录因子的功能分析中得到了广泛应用。以玉米转录因子zmCBF1与顺式元件CRT的结合为例,建立了用于转录因子分析的GRA/EMSA实验体系,并对其在应用中可能出现的问题进行了分析。

Gel Retardation Assay （GRA） is an effective method to identify the interaction between proteins and nucleic acids, which is applied to the function analysis of transcription factors. In the present study, we established the GRA system for analysis of Maize transcription factor zmCBF1 interacting with the cis element CRT.