人肝癌c─myc基因产物和HBxAg表达研究

Study on the expression of c-myc gene product and HBxAg in human hepatocellu-lar carcinoma

本实验对４８例石蜡包埋人肝癌和癌旁组织的Ｐ６２￣（ｃ－ｍｙｃ）和ＨＢｘＡｇ进行了免疫组化（ＡＢＣ法）检测。结果显示，Ｐ６２￣（ｃ－ｍｙｃ）在肝癌和癌旁组织阳性率分别为８３．３％（４０／４８）和８９．６％（４３／４８），肝癌及其癌旁组织ＨＢｃＡｇ阳性率均为９３．８％（４５／４８）．在肝癌组织中Ｐ６２￣（ｃ－ｍｙｃ）与ＨＢｘＡｇ的表达呈显著正相关（Ｐ＜０．０５），这为ＨＢｘＡｇ在肝癌中作为叵式作用因子激活ｃ—ｍｙｃ癌基因提供了细胞水平的证据。ｃ－ｍｙｃ基因过度表达参与了人肝癌的发生发展过程。

Immunohistochemical technique(ABC) was used to detect the expression of p62￣(c-myc) and HBxAg inparaffin embedded tissues of human hepatocellular carcinoma(HCC)and corresponding adjacent non-tumor-ous liver tissues (NT)from 48 patients.The results showed that the positive rates of p62￣(c-myc) were 83.3%(40/48)in HCC and 89.6%(43/48)in NT.The positive rates of HBxAgwere 93.8%(45/48)in HCC andNT,The significant positive correlation was noticed between p62￣(c-myc) and HBxAg in HCC(P<0.05),givingfurther evidence that HBxAg might function as a transactivator in the course of c-myc proto-oncogene activa-tion,These observations suggest that over expression of c-myc gene may be closely associated with hepato-carcinogensis.