表皮生长因子受体在胃癌中的表达及其意义

High expression of epidermal growth factor receptor in gastric carcinoma and itssignificance

表皮生长因子受体（ＥＧＦＲ）是分子量为１７０ｋｕ的跨膜糖蛋白，与细胞的分裂增殖有关。本研究应用放射配基结合分析法，测定了３０例胃癌患者的癌组织及正常胃组织，结果胃癌组织的ＥＧＦＲ含量为（１０．３６±２．２１）ｆｏｍｌ／Ｌ蛋白，明显高于正常胃组织的ＥＧＦＲ含量（４．２２±１．８０）ｆｍｏｌ／ｍｇ蛋白，（Ｐ＜０．０１）。不同胃癌病理类型中，分化差的ＥＧＦＲ高于分化好的，但统计学处理无显著性差异。胃癌组织中的ＥＧＦＲ具有很高的亲和力Ｋｄ＝（２．２５±０．６８）ｎｍｏｌ。我们的结果表明ＥＧＦＲ在胃癌组织中的高表达可能在胃癌的发生、发展中起重要作用，但ＥＧＦＲ能否作为判断胃癌分化好坏及预后的指标尚难定论，有待进一步研究。

Thirty cases of gastric carcinomas were studied by radioligand binding assay for determing epider-mal growth factor receptor(EGFR).The difference of EGFR expression in malignant and normal tissues andthe difference of EGFR expression among several pathological types of the carcinoma were evaluated.The re-sults showed that the level of EGFR in the cancer specimens,was significantly higher than that in the normalspecimens,10.36±2.21 fmol/mg protein vs.4.22±1.80 fmol/mg protein(P<0.01).The constant of disso-ciation (Kd) of cancer specimen was higher than that of normal tissues,2.25±0.68 vs,0.74±0.56 nmol(P<0.01).Taking different histological types into account,the EGFR in poorly-differentiated adenocarcinomasshowed a tendency of increased level compared with that in well-differentiated adenocarcinomas,but there wasno statistical significance,The results suggest that EGFR in neoplastic tissues have a higher affinity to EGFRand much more active biological reaction takes place in the gastric neoplasm under the same levels of EGFR. The high level of EGFR in gastric carcinoma should play an important role in the developement and progressof gastric carcinomas.However, the relationship between the expression of EGFR and clinical prognosis ofgastric carcinoma need to be further studied in the future.