乙醛－氨基糖甙类抗生素的1·5次微分电分析

1.5 Order Differential Electroanalysis of Acetaldehyde-aminoglycoside Antibiotics

于Ｂｒｉｔｔｏｎ－Ｒｏｂｉｎｓｏｎ－ＣＨ３ＣＨＯ体系中，采用１．５次微分测定了阿米卡星（含伯氨基）、小诺霉素（含仲氨基）等氨基糖甙抗生素，检测限为２．８×１０－７ｍｏｌ／Ｌ。同时用不同的电化学方法对该类抗生素在悬汞电极上的吸附特征和电极过程作了探讨，测得控制电极反应速度步骤的电子数为２，氢离子数为１，从而导出其反应方程式。方法用于尿、血清及软膏样品中痕量该类抗生素的测定，回收率均在９０％～１１５％之间。

The 1.5 order differential electro-analytical behaviour of aminoglycoside antibiotics has been investigated in the medium of the Britton-Robinson's buffer solution (pH 10. 2) and ac-etaldehyde then the mechanism of its electrode process has been discussed. It was found that the product of antibiotics reacted with CH3CHO had adsorptive character and its irreversible re-duction peak potential was observed at - 1.4V. In the optimum conditions the peak current increased linearly with the increase of the concentration of aminoglycoside antibiotiace in the range of 2. 8× 10-7～1. 4 × 10-5 mol/L and its related coefficient was 0. 9992. This method was applied in the determination of trace aminoglycoside antibiotics in blood serum, ungent without pretreatment and recovery was 90 %～115 %.