有机碳源和溶解氧对亚硝酸盐生物硝化的影响研究

Effects of organic substrate and dissolved oxygen on nitrification process of nitrate

从城市污水处理厂的活性污泥中纯化分离到高活性硝化菌株N-20(Nitrobacter sp.),在一种新型材料Carbon Foam的表面挂膜;通过对菌株N-20进行摇瓶试验,选择添加有机碳源的种类,将其在pH 7.5～8.0、温度28℃的条件下,分别以NaNO2和K2HPO4为氮源和磷源,通过生物滤塔中的液相连续试验,考察在不同DO的条件下有机碳源对硝化作用的影响规律.结果表明,以葡萄糖作为有机碳源,DO≥2 mg/L,葡萄糖低于20 mg/L时,生物滤塔内可进行正常硝化,NO2-- N的硝化去除率维持在90%左右,随着葡萄糖浓度的增加,硝化去除率下降到70%;DO≤2 mg/L,葡萄糖对硝化作用的抑制增强,当葡萄糖为200 mg/L时,生物滤塔中NO2--N的硝化去除率仅为32%.

N-20 （Nitrobacter sp. ） was acclimated from facility＇s secondary sludge over the surface＂ of a new kind of material-carbon foam. The effect of organics in nitrification process under different DO was examined under the conditions of pH 7.5-8.0, temperature 28 ℃, NaNO2 as the nitric source, K2HPO4 as phosphorous souree. The test was carried out continuously in a biofilter. The results show that nitrification process could be carried out normally with a NO^2--N removal of 90% under the conditions of 20 mg/L glucose as organic substrate, DO≥2 mg/L, and NO2^--N removal decreased to about 70% when the concentration of glucose increased. When DO≤2 mg/L, role of glucose on the nitrification process would be refrained, and NO2^--N removal was only 32% when the concentration of glucose was 200 mg/L.