莲房原花青素对^(60)Co-γ射线致亚急性辐射损伤防护的研究

STUDY ON PROTECTION OF PROANTHOCYANIDINS FROM LOTUS SEEDPOD ON SUBACUTE DAMAGE CAUSED BY ^(60)Co-γ RADIATON

目的:研究莲房原花青素(LSPC)的抗辐射作用。方法:80只小鼠分4组,实验组分别连续灌胃不同剂量(50、100、200mg/kgbw)的LSPC15d后,接受60Co-γ射线8.0Gy照射后,一半动物观察平均存活时间和30d存活率。另一半动物于照射后不同时间检测外周血白细胞、红细胞、血小板、血红蛋白含量。结果:小鼠经8.0Gy60Co-γ一次全身辐照后,100、200mg/kgbwLSPC组能使小鼠平均存活时间分别提高49.2%和58.3%,存活率明显高于对照组,且各组小鼠的保护指数均大于1.0;外周血像于照后21d基本恢复正常,且以200mg/kgbwLSPC组效果最佳。结论:LSPC对8.0Gy60Co-γ射线亚急性辐射损伤有一定的保护作用。

Objective： To investigate the antiradiation effect of lotus seedpod proanthocyanidins （LSPC）. Method： Eighty mice were given different dosages （50, 100,200 mg/kg bw） of LSPC continuously for 15 d and then exposed to 8.0 Gy ^60Go-γ radiation, and half of the mice were observed for average survival time and 30 d survival rate, another half assayed for peripheral leucocyte, erythrocyte, platelet, and hemoglobin contents at different time after radiation. Results： After mice were exposed to 8.0 Gy ^60Go-γ, average survival time of 100, 200 mg/kg bw LSPC groups increased by 49.2% and 58.3% respectively, and the survival rates were remarkably higher than the control group, and the protective index of each group was above 1.0. The peripheral hemotological picture restored normal in 21 d, and 200 mg/kg bw LSPC group had better recovery. Conclusion： LSPC can protect mice from subacute damage caused by 8.0 Gy ^60Go-γ radiation.