莲房原花青素对乙醇诱导肝细胞损伤的拮抗作用

THE PROTECTIVE EFFECT OF LOTUS SEEDPOD ON HEPATOCYTE DAMAGE INDUCED BY ETHANOL

目的:研究莲房原花青素(LSPC)对乙醇诱导的人胚肝细胞株L-02损伤的拮抗作用。方法:观察5、10、25mg/L的LSPC与200mmol/L乙醇共同作用于肝细胞L-0224h后,对细胞的生存率,超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)含量、凋亡率以及DNA损伤等指标的影响。结果:与乙醇组相比,5mg/L和10mg/LLSPC使细胞的生存率由73.14%上升到84.79%和90.52%,提高了细胞SOD、CAT、GSH的含量(P<0.01),降低MDA的生成;有效降低细胞的凋亡率,降低细胞p53和生长抑制DNA损伤基因(GADD)34、45β、153的表达,但高剂量组(25mg/L)对乙醇的拮抗作用不明显。结论:适量浓度的莲房原花青素对乙醇诱导的肝细胞L-02的损伤有拮抗作用。

Objective： To study the protective effect of lotus seedpod procyanidins （LSPC） on ethanol-induced damage to human embryo liver L-02 cells. Method： L-02 cells were exposed to 200mmol/L ethanol, 200mmol/L ethanol ＋ 5, 10 and 25 mg/L LSPC respectively for 24 h. Survival rate, levels of superoxide dismutase（SOD）, glutathione （GSH）, catalase （CAT）, malonyldiadehyde （MDA）, apoptosis rate and the expression level of DNA damage genes were measured. Results： Compared with the ethanol group, the L-02 cell survival rate of 5 and 10 mg/L LSPC group increased from 73.14% to 84.79% and 90.25% respectively, SOD, GSH, CAT levels increased, MDA level, apoptosis rate, the expression level of p53 and growth arrest and DNA damage-inducible genes （GADD） decreased. But the same effect wasn＇t observed in 25 mg/L LSPC group. Conclusion： 5 mg/L and 10 mg/L LSPC can protect ethanol induced damage in human embryo liver L-02 cells.