cDNA微阵列技术分析nm23在肺鳞癌中的表达

cDNA Microarrays Analysis of nm23 Expression in Lung Squamous Cell Cancer

【目的】利用cDNA微阵列技术研究Ⅰ期肺鳞癌基因表达谱,重点分析nm23基因在肺鳞癌组织中的表达概况。【方法】采用6个分期为Ⅰ期的手术切除鳞癌标本与6张含4 096个基因的人基因表达谱芯片,将来源于肿瘤细胞与正常肺组织的RNA进行配对,用不同的荧光染色剂进行逆转录标记成cDNA探针后混合,与芯片上的基因进行杂交,扫描两者的信号强度,得出某一基因在肿瘤组织与正常肺组织的荧光比值,将比值大于2称为表达上调或过度表达,比值低于0.5的称为表达下调或低表达。得到6个肺鳞癌基因表达谱并对其进行比较,找出6个基因表达谱中上调或下调的基因。【结果】nm23在全部6个肺鳞癌标本中均表达上调。【结论】nm23在早期肺鳞癌中出现表达,提示其可能为肺鳞癌的分子标志物,为进一步研究肺鳞癌发生、发展的分子机制及预后提供线索。

[Objective]To identify nm23 expression in early stage lung squamous cell carcinoma by cDNA microarrays. [Methods]Six surgical specimens and 6 microarray chips each containing 4096 human target genes were used for the current study. The pathologic stage was I （AJCC） in six tumors, six tumor specimens were lung squamous cell carcinomas. Paired mRNAs from carcinoma cells and normal lung tissue specimens from the same lobe were labeled with different fluorochromes during cDNA probe synthesis in a reverse-transcription reaction. Both synthesized, labeled cDNA probes were mixed and hybridized to the microarray. The signal intensity of each spot was measured by laser scanner and gene expression was quantified as the tumor-to-normal fluorescence ratio （T： N ratio）. The gene was up-regulated （overexpressed） when the T： N ratio was greater than 2.0 and down-regulated （underexpressed） when the ratio was less than 0. 5. While thses standards were used to identity gene expression profiles in lung squamous cell carcinoma, 6 gene expression profiles were obtained and further to identify the same expressed gene in them. [Results]nm23 was overexpressed in all the 6 lung squamous cell cancer tissues. [Conclusion]nm23 is overexpressed in early stage lung squamous cell carcinoma, suggesting that it is possible moleucular markers and important fator of carcinogenesis, invasion, metastasis and prognosis of lung squamous cell carcinoma.