HPLC法同时测定红直獐牙菜中3种有效成分的含量

Simultaneously determination of three effective components in Swertia erythrotictaMaxim by HPLC

建立了反相高效液相色谱法(HPLC)同时测定红直獐牙菜中的獐牙菜苦苷、龙胆苦苷、芒果苷含量的方法.在检测波长254 nm、柱温30℃、流速1 mL/min时,用ZORBAX SB-C18(250mm×4.6mm i.d.,5μm)柱,以MeOH和H2O(含0.04%H3PO4)在0～20 min内由 20%至35%线性梯度洗脱.上述3种成分均达到基线分离,獐牙菜苦苷、龙胆苦苷、芒果苷的线性范围分别为0.0095～2.9μg(r=0.9999),0.0486～2.56μg(r=0.9999),0.0056～2.8μg(r=0.9999);回收率为97.7%(RSD=4.3%),99.5%(RSD=3.5%),103%(RSD=1.1%).

To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside and mangiferin in Swert/a erythroticta Maxim by RP - HPLC. The samples were separated on the column of Zorbax SB- C18 （250 mm × 4.6 mm i.d., 5μm） which were diluted with methanol and water （0.04% phosphoric acid）.The ratio of methanol and water increased from 20：80 to 35：65 in 20 min with a detected wavelength of 254 nm; flow rate of 1 mL·min^-1 ;column temperature of 30℃. Three compounds were base - isolated. The linear ranges of swertiamarin, gentiopicroside and mangiferin were 0.0095 - 2.9 μg（r = 0.9999） ,0.0486 - 2.56 μg（r = 0.9999） ,0.0056 - 2.8 μg（r = 0.9999） respec- tively;The average recoveries were 97.7% （RSD = 4.3%）, 99.5% （RSD = 3.5%）, 103% （RSD = 1.1% ） respectively.