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17β-雌二醇对子宫内膜间质细胞体外分泌趋化因子IL-8、MIP-1α、RANTES和I-309的影响 被引量：3

Effect of 17β-estradiol on secretion of the chemokines IL-8,MIP-1α,RANTES and I-309 by endometrial stromal cells in vitro
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摘要 目的探讨17β-雌二醇对子宫内膜间质细胞分泌IL-8、MIP-1α、RANTES和I-309的调控,以解析雌激素在子宫内膜异位症发生发展中的作用机制。方法用不同浓度17β-雌二醇处理子宫内膜间质细胞48小时;此后选用10-9mol/L17β-雌二醇处理子宫内膜间质细胞12、24、36和48小时,ELISA法分析刺激前后培养上清中IL-8、MIP-1α、RANTES和I-309分泌水平。结果子宫内膜间质细胞体外高水平自然分泌IL-8,17β-雌二醇能抑制IL-8的分泌,呈剂量依赖性。RNATES和MIP-1α分泌水平较IL-8低,经雌激素刺激子宫内膜间质细胞后MIP-1α表现出与IL-8相同的分泌方式,但对RANTES分泌无明显影响。子宫内膜间质细胞经雌激素刺激前后均未检测到I-309。结论雌二醇可能参与子宫内膜白细胞和单核巨噬细胞的募集,但不是异位灶中上述趋化因子高表达的原因。 Objective： To elucidate the role of estradiol in the pathogenesis and development of endometriosis by exploring the effects of 17β-estradiol on IL-8,MIP-1α, RANTES and I-309 secretion by endometrial stromal cells.Methods：IL-8,MIP-1α, RANTES and I-309 protein in culture supematant of endometrial stromal cells after treated by 17β-estradiol with different concentrations for 48 h or for 12-48 h with 10^-9 mol/L were quantified using EIASA kits of R＆D systems.Results：The endometrial stromal ceils in vitro secreted spontaneously more IL-8 than MIP-1α and RANTES.The estradiol inhibited IL-8 and MIP-1α secretion in a concentration-dependent manner（ P 〈 0.05）,but did not affect RANTES secretion.Human endometrial stromal cells did not secrete the chemokine I-309 protein whether treated with estradiol or not. Conclusion ： The estradiol may recruit endometrial macrophage and leukocytes migration by regulating IL-8 and MIP-1α secretion of endometrial stromal cells,and alone is not account for the increased expression of IL-8, RANTES and MIP-1α in the ectopic tissue of endometriosis.
作者 史颖莉 李大金 华克勤 朱影 王明雁 袁敏敏 孟毅
机构地区 复旦大学上海医学院附属妇产科医院暨妇产科研究所
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2005年第12期928-931,共4页 Chinese Journal of Immunology
基金 复旦大学"985"项目资助(985B36)
关键词 子宫内膜间质细胞 17Β-雌二醇 趋化因子 ELISA法 17β-estradiol Endometrial stromal cells Chemokine ELISA
分类号 R711.11 [医药卫生—妇产科学]
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参考文献13

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同被引文献36

  • 1罗雪珍,史颖莉,李大金,华克勤,朱影.子宫内膜及异位灶趋化因子受体转录在子宫内膜异位症发病中的作用[J].中国免疫学杂志,2005,21(9):705-709. 被引量：12
  • 2Shi YL, Luo XZ, Zhu XY, et al. Effects of combined 17β- estradiol with TCDD on secretion of chemokine IL-8 and expression of its receptor CXCR1 in endometriotic focus-associated ceils in co-culture. Hum Reprod 2006 ; 21 (4) : 870-879.
  • 3Shi YL, Luo XZ, Zhu XY, et al. Combination of 17β-estradiol with the environmental pollutant TCDD is involved in pathogenesis of endometriosis via up-regulating the chemokine 1-309/ CCR8. Fertil Steril 2007 ; 88(2) : 317--325.
  • 4Yu J, Wang Y, Zhou WH, et al. Combination of estrogen and dioxin is involved in the pathogenesis of endometriosis by promoting chemokine secretion and invasion of endometrial stromal cells. Hum Reprod2008; 23(7): 1614-1626.
  • 5Rier SE, Martin DC, Bowman RE, et al. Endometriosis in rhesus monkeys (Macaca mulatta) following chronic exposure to 2, 3, 7, 8 - tetrachlorodihenzo - p - dioxin.Fundamental and Applied Toxicology, 1993, 21:433 -441.
  • 6Chen ZH, Hurh YJ, Na HK, et al. Resveratrol inhibits TC- DD- induced expression of CYP1A1 and CYP1B1 and catechol estrogen - mediated oxidative DNA damage in cultured human mammary epithelial cells. Carcinogenesis, 2004, 25: 2005 - 2013.
  • 7Hombach - Klonisch S, Pocar P, Kauffold J, et al. Dioxin exerts anti-estrogenic actions in a novel dioxin - responsive telomerase- immortalized epithelial cell line of the porcine oviduct (TERT- OPEC). Toxicological Sciences, 2006, 90: 519 - 528.
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中国免疫学杂志
2005年 第12期

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