新型乙酰胆碱酯酶抑制剂对阿尔茨海默病大鼠行为记忆的作用

Effect of novel AChE inhibitor on spatial learning and memory of Alzheimer rats

目的探讨新型乙酰胆碱酯酶抑制剂(novel AChE inhibitor,NAI)对阿尔茨海默病(AD)大鼠空间记忆行为的影响,为AD病人的临床治疗提供可信的行为学资料.方法实验选用48只3～5月龄Wistar雄性大鼠,随机分为4组(n=12):NAI组、石杉碱甲(Hup) 组、单纯损伤(SO)组,空白对照组,将前3组大鼠切断穹隆-海马伞制作AD动物模型,然后分别进行Morris水迷宫实验.结果定位航行实验中,NAI组寻找水下平台的逃避潜伏期较SO组显著缩短(P＜0.01).空间探索实验中,各象限游泳距离占总距离百分率和跨越各象限平台相应位置次数占总次数百分率,NAI组均较SO组明显增高 (P＜0.01).结论 AD大鼠经过NAI治疗及水迷宫行为训练后,可显著改善其对空间学习记忆的能力,提示NAI对AD大鼠具有一定的治疗作用.

Objective To investigate the effects of novel AChE inhibitor （NAI） on spatial learning and memory of Alzheimer disease （AD） rats and provide credible behavioural data for clinical treatment of AD. Methods 48 male Wistar rats aged 3 - 5 months were averagely randomly divided into NAI, huptrzin A （ Hup）, simple-operated and control group, the rats in the former three groups were cut off fornix-fimbria hippocampus to make AD models. All groups were tested on Morris water maze test. Results The escape latency of NAI group was significantly shorter than that of SO group （ P 〈 0.01 ） in the place navigation test. The percentages of swim distance in the quadrant and times of crossing the platform of NAI group significantly increased than those in SO group （ P 〈 0.01 ） in spatial explore test. Conclusions NAI treatment and water maze training could promote the ability of spatial learning and memory of AD rats, which suggest that NAI should play a therapeutic role in AD.