摘要
将柑桔幼胚分离接种于MS固体诱导培养基上诱导了体细胞胚直接高频发生并再生植株,初步建立了柑桔体细胞胚直接高频发生的实验系统。研究结果表明,柑桔幼胚在附加1mg/LIAA和20mg/LBA的MS培养基上的直接体细胞胚诱导频率最高。
Using immature zygotic embryos of Citrus as explants, primary somatic embryogenesis in vitro was directly induced with high frequency, and plantlets were regenerated.A preliminary regeneration system was established in this study. The results showed that the induction frequency of directly induced somatic embryos is highest when immature zygotic embryos are cultured on MS medium supplemented with 1mg/LIAA and 20mg/LBA.
出处
《河南科技大学学报(自然科学版)》
CAS
2005年第6期82-84,共3页
Journal of Henan University of Science And Technology:Natural Science
基金
福建省自然科学基金资助项目(C9810021)
关键词
柑桔
幼胚培养
体细胞胚
Citrus
Immature zygotic Embryo culture
Somatic embryogenesis