阿托伐他汀改善心室重构作用及其抗氧化机制研究

The Effect of Atorvastatin on Improving Ventricular Remodeling and its Mechanism

目的研究阿托伐他汀改善心室重构作用及其抗氧化机制。方法选用16周龄雄性自发性高血压大鼠(SHR)18只,随机分为3组,每组6只:阿托伐他汀组、阳性对照组(缬沙坦组)和阴性对照组(SHR组),另选同周龄雄性Wistar-Kyoto大鼠(WKY)6只作为正常对照组(WKY组),给药6周后测定全心重量(HW)、左心室重量(LVW)、左心室重量指数(LVWI)、心肌羟脯氨酸含量和胶原蛋白含量,透射电镜观察心肌超微结构,同时检测血清和心肌组织超氧化物化酶(SOD)活力、丙二醛(MDA)含量。结果给药6周后,阿托伐他汀组HW、LVW、LVWI、心肌羟脯氨酸含量和胶原蛋白含量明显低于SHR组(P<0.05);电镜显示阿托伐他汀组心肌与SHR对照组比较,肌原纤维较清晰,排列整齐,横纹清楚,间质胶原纤维无明显增生;阿托伐他汀组血清和心肌组织SOD活力明显高于SHR组、MDA含量明显低于SHR组(P<0.05)。结论阿托伐他汀有明显改善心室重构的作用,其机制可能与其抗氧化作用有关。

Objective To study the effect of atorvastatin on improving ventricular remodeling and its mechanism. Methods Eighteen 16 - week- old male spontaneously hypertension rats （SHR） were randomly divided into three groups. Each group has six rats： atorvastatin group, positive control group （valsartan group） and negative control group （SHR group）. Besides, six same old male Wistar- Kyoto rats （WKY） were served as normal control group. All rats in atorvastatin group and valsartan group were treated with atorvastatin or valsartan for 6 weeks, respectively. Then the entire heart weight （HW）, left ventricle weight （LVW）, left ventricle weight index （LVW/）, flae levels of myocardial hydroxyproline and collagen protein in three group＇ s rats were measured. Ultrastructure of cardiac muscle was observed with transmission electron microscope. The levels of serum and myocardial SOD, MDA were also measured. Results After 6 weeks treatment, HW, LVW, LVWI, the levels of myocardial hydroxyproline and collagen protein in atorvastatin group were significantly lower than those in SHR group （P 〈 0.05）. Electron microscope displayed that compared with SHR group, myofihrils were clearer, their rank was orderly, transverse striations in them were clear and interstitial collagen fibers didn＇t obviously hyperplasy in cardiac muscle of atorvastatin group. The levels of serum and myocardial SOD in atorvastatin group were significantly higher, while the levels of serum and myocardial MDA were signiiicantly lower than those in SHR group （ P 〈0.05）. Conclusions Atorvastatin can improve cardiac ventricle remodeling obviously, the mechanism of which may be related with antioxidation.