利用微波诱变技术提高菌株B1抑菌活性的研究

Effects of mutation induced by microwave irradiation on antifungal activity of marine strain B1

目的研究微波辐射技术对具强抑菌活性的海洋微生物紫外突变株B1的诱变效应,并获得诱变处理的合适剂量。方法对处于对数生长期的菌悬液进行不同时间微波辐射处理,基于微生物诱变育种随机性,利用数理统计方法对诱变结果分析评估,并对筛选的高活性菌株进行传代。结果60s微波辐射为最适诱变剂量,在此处理上获得的1株高活性橙色菌株B1-413,其最小抑菌浓度(MIC)由亲代B1的125μl.mL-1,降低至突变株B1-413的32μl.mL-1,抑菌活性提高了近4倍,并经传代实验证实其抑菌活性稳定。结论菌株B1对微波辐射敏感,诱变效应明显。

Objective To study the effect of microwave irradiation on a marine mutant strain B1 which had high antifungal activity , and obtain the optimum dosage of microwave irradiation. Methods The marine strain Bl（Bacillus sp. ） was radiated by 2450 MHz microwave for various times. The mutation rate p, mutation mean μ and the mutation variance σ^2 were estimated by statistical methods. From these mutants, a high-activity strain B1-413 was obtained, and its genetic attribute was tested . Results Microwave irradiation was highly effective at 60 s. The MIC of mutant strain B1-413 was reduced from 125μl · mL^-1 to 32μl· mL^-1 compared with B1, the genetic attribute of which was stable through four generation cultivation. Conclusion The B1 was sensitive to microwave irradiation, and the higher anti-fungi active mutants can be easy obtained after the treatment of microwave irradiation.