海藻糖对液氮保存同种瓣的保护作用

Cryoprotective effect of trehalose in keeping aortic valve homograft

目的探索将海藻糖作为保护剂用于液氮冻存同种瓣。方法10%二甲亚砜+0.1mol.L-1海藻糖(实验组)和单独使用10%二甲亚砜(对照组)作为冷冻保护剂,经程控梯度降温,液氮冻存大鼠的同种瓣6个月后复温,通过电镜、光镜观察和内皮细胞活力测定,对两种保存方法的优劣进行比较。结果实验组的内皮细胞活力明显高于对照组,并且其结构的破坏也较对照组轻。结论10%二甲亚砜+0.1mol.L-1海藻糖作为同种瓣的冷冻保护剂,其保护效果明显好于单独使用10%二甲亚砜。

Objective To search for the application of trehalose as cryoprotectant for keeping aortic valve homograft in liquid nitrogen. Methods The aortic valve homografts of Wistar rat were kept in 10%DMSO and divided into 2 groups（control and test group）. The test group was added a supplement of trehalose 0.1mol· L^-1 and all the hemografts were gradually hypothermied and then cryopreserved in-196℃ liquid nitrogen. After 6 months all the cryopreserved valves were thawed. The aortic valve homografts were observed by light and electron microscope. At the same time, the viability of endotheliocyte was measured. Results The viability of endotheliocyte in test group was higher than that in the control group and the structural damage was also much lighter. Conclusion The cryoprotective effect of combined use of 10%DMSO＋0.1mol·L^-1 trehalose was much better than that of 10%DMSO alone.