摘要
建立体外培养的小鼠胸腺淋巴细胞紫外线(UV)辐射损伤的病理模型,采用MTT法检测细胞活性;酶生化法测定胞浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、活性氧(ROS)、丙二醛(MDA)含量以及细胞抗超氧阴离子能力(A-SAC)和总抗氧化能力(T-AOC);流式细胞仪Rhodamine 123荧光染色测定线粒体膜电位(ΔψМ)等方法,探讨扇贝多肽(PCF)对小鼠胸腺淋巴细胞辐射损伤的保护作用及机制。结果显示PCF能显著提高小鼠胸腺淋巴细胞的活性;抑制紫外线辐射所致淋巴细胞的损伤,显著提高细胞浆中SOD、GSH-Px的活性,降低ROS,MDA含量,提高A-SAC及T-AOC;稳定线粒体的膜电位;表明扇贝多肽对紫外线辐射损伤的小鼠胸腺淋巴细胞具有保护作用。其作用机制与PCF能清除氧自由基、提高抗氧化酶活性,保护细胞膜组织结构有关。
To study the protective effects of PCF on thymocytes in the mice damaged by ultraviolet ray in vitro, The irradiation damage model of thymocytes was established. The indices of GSH-Px, SOD, MDA, T-AOC and the cell viability were determined by biochemical and MTT methods respectively. The mitochondria membrane potential was tested using flow eytometry （FCM）. The results showed that PCF could enhance the activities of GSH-Px, SOD and increase the values of A-SAC and T-AOC in cells, while decrease the amounts of ROS, MDA. In addition, PCF could stabilize the mitoehondria membrane potential. These results indicated that PCF had the protective effects on the thymocytes damage by UV irradiation, The mechanisms of the effects might be related to the elimination of oxygen free radical and increase of the activities of anti-oxidative enzymes in ceils.
出处
《中国海洋药物》
CAS
CSCD
2005年第6期28-31,共4页
Chinese Journal of Marine Drugs
基金
青岛市科技局资助项目(No.04-2-SH-692000-260)
关键词
扇贝多肽
淋巴细胞
紫外线
抗氧化
polypeptides from Chlamys farreri
lymphocyte
ultraviolet ray
antioxidation
