缺氧诱导因子对肝癌细胞生长和侵袭能力的影响

The effects of hypoxia inducible factor-2α on the proliferation and invasion of the hepatocellular carcinoma cells

目的检测在缺氧条件下缺氧诱导因子2α对肝癌SMMC-7721细胞生长和侵袭能力的影响。方法缺氧培养条件下脂质体Lipofectamine介导缺氧诱导因子（HIF-20α）脱氧寡核苷酸转染SMMC-7721肝癌细胞，测转染前后HIF-2α mRNA量和蛋白量检验转染效果；MTY法测定细胞生长和黏附能力变化；Transwell小室测定细胞的体外侵袭能力。结果HIF-2αmRNA和蛋白表达在反义转染组显著低于正义组、随机组与对照组（P〈0．01）。HIF-2仪表达阻断对细胞生长和活力无显著性影响（P〉0．05），但显著抑制体外粘附能力（抑制率16．99％～31．07％）和侵袭能力（抑制率17．9％）（P〈0．01）。结论HIF-2α表达阻断可抑制SMMC-7721肝癌细胞的黏附和侵袭活性。对其生长无显著影响。

Objective To investigate the effects of hypoxia inducible factor- 2α （HIF -2α） on the proliferation and invasion of hepatoeellular carcinoma cell line SMMC -7721 under the anoxia condition. Methods Lipofeetamine - mediated HIF -2α oligodeoxynueleotide （ODN） was transfeeted into SMMC -7721 cells and the expressions of HIF- 2α mRNA and protein were detected to evaluate the transfeetion, The proliferative and adhesive activity was determined by methyl thiazolyl tetrazolium （MTF） method and the invasive activity was determined by a transwell cell culture chamber method. Results cantly by HIF- 2α antisense oligodeoxyribonueleotide （ASODN） （ P The expressions of HIF - 2α mRNA and protein were inhibited signifi- 〈0.01 ）. The blockage of HIF- 2α expression significantly inhibited the adhesive activity （ inhibition ratio 16. 99% - 31.07% ） and invasive activity （ inhibition ratio 17.9% ） （ P 〈 0. 01 ）, with no significant influence on the proliferative activity of SMMC - 7721 cells （ P 〉 0. 05 ）. Conclusion The blockage of HIF - 2α expression inhibits the ad- hesive and invasive activity of SMMC -7721cells, and has no evident impact on their proliferation.