安菜的组织培养与植株再生

Tissue Culture and Plant Regeneration of Vicia Kulingiana Baily

通过对安菜组织培养的培养基种类、激素配比、碳源的研究,得出适于安菜侧芽分化生长的最佳培养基为MS+6-BA0.5mg/L+NAA0.2mg/L。诱导愈伤组织以2,4-D浓度在0.2mg/L^0.5mg/L之间最佳。碳源以蔗糖,浓度3%最佳。以IBA0.5mg/L^1.5mg/L浓度诱导生根效果最好。

Studied on midum kind,hormones combination, sugar concentration of Vicia Kulingiana aily was cenducted. The result showed that the best medium of inducting lateral shoot was MS ＋ 6-BA0.5 mg/L ＋ NAA0.2 mg/L. The optimal hormones of inducting callus was 2,4-D 0.2 mg/L ～ 0.5mg/L. The optimal sugar concentration was 3% sucrose. The best rooting medium was MS ＋ IBA0.5mg/L ～ 1.5 mg/L.