摘要
目的建立发色底物法测定注射用降纤酶类凝血酶的方法.方法以Be-Phe-Val-Arg-PNA为发色底物,检测波长405nm.结果降纤酶在2～10单位/mL范围内呈良好的线性关系,r=0.9993(n=5).结论本法简便,准确,专属性强,适用于产品的质量控制.
OBJECTIVE A chromogenic substrate method was developed for the determination of thrombin-like in defibrase for injection. METHODS Chromogenic substrate was Be-Pbe-Val-Arg-PNA. The detection was made at 405nm. RESULTS The calibration curve of thrombin-like had good linear relationship in the range of 2 ～10unit/mL （ r = 0. 9993, n = 5）. CONCLUSION The developed chromogenic substrate method is simple,accurate and specific, and could be used as the quality control for thrombin-like in defibrase for injection.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2005年第6期482-483,共2页
Chinese Journal of Modern Applied Pharmacy
基金
广西壮族自治区自然科学基金资助项目(0342002-4)
关键词
降纤酶
类凝血酶
发色底物
含量测定
defibrase
thrombin-like
chromogenic substrate
determination
